Porvair Sciences Chromatrap
- Antibodies and Primers for ChIP assays
- ChIP-Seq Kits
- ChIP-Seq HT 96-well Plates
- Chromatrap DNA Extraction
- Chromatrap Homogenizer Spin Columns
- DNA-Kits for Extraction, Purification, Concentration and Size Selection
- gDNA Removal prior to RNA Extraction
- Specialised ChIP Kits – Native
- Specialised ChIP Kits – FFPE
- Specialised ChIP Kits – Drosophila
- Shearing Kits
- UniqSeq Kits
Solid-State Chromatin Immunoprecipitation
Chromatin immunoprecipitation (ChIP) with the patented bead-free technology from Chromatrap® provided from Porvair Sciences utilizes filters with an inert porous polymer matrix coupled with Protein-A or Protein-G on the surface. This increased surface area maximizes immunocapture of antibody-chromatin complexes from your samples.
Features:
- Easy to use and simple spin column protocol with less manual handling
- Highest sensitivity and binding capacity through optimally oriented Protein-A/G arrangement across filter (see Figure)
- No sample loss as antibody-chromatin complexes are captured and retained on filter
- Lowest background and exceptional signal-to-noise ratio compared to other ChIP kits
- Lowest amount input material, highest DNA yield
- ChIP from as low as 1000 cells, 50 ng chromatin or 5 mg tissue
- Availabe as Protein-A/G spin columns or 96 well filter plates
- Chromatin-to-Sequencing ready in under 5 hours
- Applications: Next Generation Sequencing, PCR, RT-PCR, Mass Spectrometry
Antibodies and Primers for ChIP assays
- Use of high-quality, specific ChIP validated antibody is essential for the success of a ChIP assay
- Positive and negative control targets
- Can be supplied with primer sets for extra validation
- Reduce time spent searching databases and designing primers
- Confidence in correct targets for antibodies
ChIP-Seq Kits
- Most sensitive kit at low cell numbers
- Can be used with:
- Cell lines, primary cells, and difficult cell types and tissues
- Low abundant targets
- Library preparation kits
- All sequencing platforms (e.g. Inc Illumina, Ion torrent, Roche)
ChIP-Seq HT 96-well Plates
- Perform up to 96 reactions simultaneously in 1 day
- Pair with automated liquid handling for even higher throughput
- Can be used for:
- Antibody validation
- Multiple antibodies
- Multiple samples
- Multiple cell types
- Multiple drug compounds/inhibitors
UniqSeq Kits
- Complete solution for ChIP and library preparation for next generation sequencing
- Compatible with all Illumina sequencers
- Low levels of duplication (< 2 %)
- High (98 %) uniquely mapped reads
- One tube reaction – End repair, A-tail & ligation in one step
- Bead free size selection
- Low input (500 pg DNA)
Specialised ChIP Kits – Native
- Only commercial method to study chromatin in its ‘native’ state
- No chemical fixation required
- Cells are in more natural ‘native’ state
- Can be used for:
- Non-fixed samples
- Histone modifications
- Transcription factors tightly associated with DNA
Specialised ChIP Kits – FFPE
- For use with formalin fixed paraffin embedded (FFPE) tissue
- Can be used for:
- Tissue samples
- Biopsies
- Difficult-to-lyse samples
- Range of different FFPE tissue (human & animal)
Specialised ChIP Kits – Drosophila
- Only commercial method to study Drosophila (fruit fly) chromatin
- Focus on Drosophila community of researchers and neuroscientists
- Uses as little as 5 flies or 500 ng chromatin
- Can be used for:
- Whole body samples
- Larvae samples
- Pupal samples
- Embryonic samples
- Histone modifications
- Transcription factors
Shearing Kits
For optimisation of shearing conditions
-->Sonication
- Simple and effective method for preparation of high-quality chromatin
- Can be used for most cell types and tissues
- Useful for difficult to lyse samples
-->Enzymatic
- Suitable for native chromatin preparation
- Effective alternative to sonication as no expensive equipment is required
- Milder treatment, can be less disruptive to the epitopes
gDNA Removal prior to RNA Extraction
The new Chromatrap® genomic DNA (gDNA) Removal Columns can be used to eliminate gDNA along with RNA extraction recommended with Chromatrap® RNA Extraction kit. This procedure ensures maximum purity and yield of pure RNA from a wide range of samples in a fast and cost-effective way.
Features of Chromatrap® gDNA Removal Columns:
- Enzyme-free gDNA removal from cell lysates within 30 seconds / sample
- Load and spin method for gDNA-free, total RNA
- Simple, one-step centrifugation method
- Highly specific gDNA removal with no loss of precious RNA
- Ideal for DNA-sensitive applications
- Can be used as an upstream gDNA removal kit prior to RNA extraction
Features of Chromatrap® RNA Extraction kit:
- Reproducible and fast extraction of high-quality total RNA
- Compatible with a wide range of samples including buffy coat, buccal swabs, and gram-negative bacteria
- Process up to 107 cultured animal cells, 50 mg animal tissue, 0.5 ml blood, 250 mg plant material, 5 x 107 yeasts or 5 x 108 bacteria
- High yield extraction of up to 1000 µg pure RNA
- Application-ready total RNA for qPCR, Microarray or other in vitro applications
Chromatrap® DNA Extraction
- Used to extract DNA from cells, tissues and blood samples
- Allows highest amount of DNA recovery from starting sample (DNA recovery up to 98 %)
- Purification of up to 50 µg DNA
- Samples are free from contaminants such as salts, inhibitors or enzymes
- For applications such as PCR, ChIP, Southern blotting, DNA methylation studies, Molecular cloning and Sequencing
- Phenol- and chloroform-free
DNA kits to extract, purify, concentrate and size select
- Simple and fast procedure
- In 96-well format for high-throughput
- DNA Extraction: high quality DNA from up to 25 mg tissue or 5 million cells in 200 µl
- DNA Purification: removal of undesired impurities from up to 50 µg DNA
- DNA Purify & Concentrate: Purify and concentrate up to 10 µg DNA in as little as 5 to 10 µl
- Size Selection: Bead-free exclusion of DNA fragments < 200 bp
Chromatrap® Homogeniser Spin Columns
Quickly and efficiently homogenise cells and tissues lysates
in a single step. Contained within a spin column, the unique
proprietary bio-shredding Vyon® polymer reduces lysate viscosity and captures insoluble debris by centrifugation.
- Perfect alternative to syringe & needle homogenisation
- Quick, clean and efficient homogenisation
- Reduces viscosity of lysate
- Ideal sample preparation for nucleic acid miniprep and midiprep
- Manufactured from PP, stable until -80 °C
- Centrifuge at max. 25,000 x g with fixed angled rotors and 30,000 x g with swing out rotors
- Maximal sample volume of 700 µl
- No cross contamination possible
Weitere Informationen zu den Produkten von Porvair Sciences können Sie hier finden:
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