Plates for the Thermo Scientific™ KingFisher™ Systems

Filtratrion Plates

• Suitable for different applications: i. e. DNA-isolation, binding assays etc.
• Polypropylene or polystyrene plates with different filtration materials such as glass wool, UHMW PE, PVDF, PES or PP
• Overview of all filter plates with possible applications available
• 48-, 96-, and 384-well plates available
• Volumes from 300 Μl up to 7.5 ml
• Long Drip and Short Drip
• Each well has an individual drip for 100 % sample transfer
• Suitable for automated systems
• Manufactured according to ANSI/SLAS standards
• For Standard- and Porvair vacuum manifolds

Krystal™ plates

• Clear bottom made of polystyrene, glass, COP (Cyclo-Olefin-Copolymer) or quartz
• COP and quartz bottom plates allow observation with wavelengths under 350 nm (down to 200 nm)
• Ideal for observation with an inverse microscope
• Fluorescence and luminescence assays
• 24-, 96-, 384-well plates
• Volumes from 120 Μl - 3.1 ml
• TC-treated and sterile versions available

Krystal™ High and Medium Bind Microplates and 8-well strips  for big molecules and proteins

Capture and detect biomolecules with uniformity, stability and reproducibility. Plates are optimised for efficient capture of a wide range of biomolecules for affinity binding assays such as ELISA`s.

• Especially interesting for COVID-19 test labs, virology and serology labs.
• Plates with medium binding capacities of 100 ng to  200 ng IgG/cm² have a hydrophobic passive adsorption of large molecules with large hydrophobic regions.
• Ideal for passive adsorption of lipid-rich biomolecules and antigens including viral antigens (non-protein).
• Plates with high binding capacities of 400 ng to 500 ng IgG/cm² have a hydrophobic surface for passive adsorption of proteins with different grades of hydrophilicity.
• Ideal for immunoassays, binding of glycoproteins and serum samples.
• Available as 96-well plate or single 8-well strips.
• 96-well plates have a total surface area of 215 mm² and  8-well strip plates of 207 mm² (both have a bottom surface area of 31 mm²).
• Conform to ANSI/SLAS standards.
• Flat bottom for superb CRISP detection.
• Working volumes up to 320 Μl.
• Ideal for high-throughput applications.

Plates for collecting and storing of liquids

Deep-Well plates

• 96- and 384-wells
• Round, square or rectangular wells
• Available in blue, black, red or white
• 220 µl – 2 ml volume/well
• Lids and sealing mats available

Deep-Well plates with low profile

Plates for various assays

• Transparent, black or white
• 24-, 96-, 384-well format
• Volumes from 30 µl - 350 µl
• Suitable for ELISA-, absorption-, flurorescence and luminescence assays
• U-, V-, flat bottom
• Round or square wells

Reservoirs

For manual and automatic applications

• Large selection
• Pyramidic bottom for minimal dead volume
• Suitable for robots
• Heat and chemically resistant (PP)
• In ANSI/SLAS standard
• Autoclavable
• Gamma irridiated
• Universal lids (made from PS) available

Seed/Plant Genomics Plates

• Ideal for determination of genomic profiles from food and commercial crops
• Suitable for extraction of plant DNA
• Hardened polypropylene to grind seeds and leaves
• Working volume 1.90 ml
• 45 mm high
• Also available sterile
• DNase- / RNase free

Solid Phase Extraction (SPE)

Porvair offers a wide range of products for Solid Phase Extraction (SPE). The solved sample can be purified and concentrated to be eluted and  then analyzed by LC-MS.

• Protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up.
• Pre-filter frit at 100 Μm traps flocculant particles >100 Μm
• Secondary frit traps fine protein particles at <10Μm
• The frits are hydrophobic and oleophobic. This retains sample/acetonitrile in the well to allow precipitation of proteins until vacuum is applied.
• Chemically inert filter material minimizes adsorption of samples.
• Frit structure prevents breakthrough of protein particles.
• PPLR frit consists of co-sintered plastics and chromatography media.
• The hydrophobic top frit prevents sample passing into the media during mixing.
• PPLR with 25 mg bed weight available as 1 ml cartridge or 96-well plate.

Microlute^® SLE for viscous liquids

• Remove hydrophilic contamination with high amounts of protein, phospholipids and salt being retained.
• Contains Diatomaceous-Earth which is known for its big pore size and a high pore volume as well as high pH-resistance (1 - 13).
• For purification of blood, plasma or serum before LC/MS analysis, environment and nutrition analysis as well as extraction of small amounts of water from hydrophobic solvents.
• Available with 200 mg or 400 mg bed weight in 96-well plate format or as 3 ml cartridge.

Microlute^® CSi and CP

Microlute^® CSi and CP take SPE to a new level of performance by enhancing the reproducibility of analyte extraction and recovery from biological, environmental and chemical samples.

Unlike traditional loose-filled SPE methods, Microlute^® CSi and CP use a hybrid structure, a solid interconnected network of evenly distributed pores combined with the retentive media (see image below). This design enhances the flow-through of samples to maximise interactions between analytes and the solid phase to deliver a reproducible SPE method that excels in performance, cleanliness and sensitivity.

• New Microlute^® hybrid technology.
• Solid interconnected network of evenly distributed pores immobilised with chromatographic media within the structure.
• Market leading reproducibility in SPE (< 4 % RSD).
• High recovery of analytes with more than 90 % recovery for acidic, basic and neutral analytes.

• Different chemistries available:
  • C18 for neutral analytes
  • Strong Cation Exchange (SCX)
  • Strong Anion Exchange (SAX)

• Available as 96-well plates with 2 mg or 10 mg bed weight/well

Combinatorial Microlute^®

• Up to 2 ml sample volume possible
• PE-bottom frits with pore sizes of 10 µm or 36 µm available
• 10 mg up to 100 mg sorbent/well compressed
• Up to 4 times faster than classic filter separation
• Plate design ideal for automation, conforms to ANSI/SLAS standards
• Long drips at the wells prevent contamination and ensure to hit the collection plate exactly
• Plate packed with bottom frits or empty
• Bottom frits are available separately
• Low dehydration and support of frits
• Also available with customized sorbents