Solid Phase Extraction (SPE)
Porvair offers a wide range of products for Solid Phase Extraction (SPE). The solved sample can be purified and concentrated to be eluted and then analyzed by LC-MS.
- Protein ‘crashes’ out of solution and precipitates directly in each well when acetonitrile is added, thus solving all common problems associated with the CRASH technique of protein clean-up
- Pre-filter frit at 100 Μm traps flocculant particles >100 Μm
- Secondary frit traps fine protein particles at <10Μm
- The frits are hydrophobic and oleophobic. This retains sample/acetonitrile in the well to allow precipitation of proteins until vacuum is applied.
- Chemically inert filter material minimizes adsorption of samples
- Frit structure prevents breakthrough of protein particles
- PPLR frit consists of co-sintered plastics and chromatography media.
- The hydrophobic top frit prevents sample passing into the media during mixing.
- PPLR with 25 mg bed weight available as 1 ml cartridge or 96-well plate
further information about MicroLute™ products from Porvair (PDF)