ECIS® Z-Theta

• Complex impedance analyser
• ECIS® (Electric Cell-substrate Impedance Sensing) quantifies morphology changes in the sub-nanometer to micrometer ranges.
• Small altering currents are applied across an electron pattern at the bottom of the array which results in a potential across the electrodes being measured by the system. Cells act as insulators increasing the impedance.
• Stimulating cells to change their function alter the impedance which can be detected by the system.
• The instrument can use a range of AC-frequencies from 100 to 100 kHz and complex impedance measurement to determine different cell morphology parameters including barrier function, close contacts, and membrane capacitance.
• 16- or 96-well station for use with 8-well or 96-well arrays
• Station is designed to put in an incubator for optimal cell growth. Incubators are also available from Dunn Labortechnik.
• Optional 8-well transfilter array station available (see below)
• Laptop (WIN 11)
• Software for data acquisition and analysis

ECIS® TEER Z

Continuous, Real-time TEER Measurement System:

• Experience seamless and repeatable label-free automated transepithelial electrical resistance (TEER) measurements, allowing you to electrically track cell layers in real-time.
• TEER in 24- or 96-well format
• TEER monitored under standard cell incubation
• Non-invasive measurement
• Read 96 wells in under 5 minutes
• Uses Millipore® or Corning® cell-culture insert plates

Specifications:

• Reads 24- or 96-well filter plates
• Medical-grade stainless steel electrode dipping pins
• 75 Hz sinusoidal excitation
• Power: < 2 watts, 12 V dc
• Station: 30 x 13 x 25 cm
• Controller: 48.3 x 43.2 x 21.6 cm
• Windows PC with ECIS® Software

User Friendly Software:

• TEER values vs. time graph
• Click-button initiation
• Colour-coded well mapping
• Stores values without cells (flat-fielding)
• Group average and compare data
• Statistical error bars
• Data output in CSV or graphical (JPEG, TIFF)

The ECIS® TEER Z Cartridge, seamlessly transitions your cell culture plate from the incubator to the cell culture hood while ensuring a sterile environment is maintained. The upper section of the plate features pairs of medical-grade stainless steel dipping pins for easy and precise insertion.

ECIS® 8-Well Transfilter Array

Benefits:

• Automated TEER measurements under incubated conditions
• Continuous monitoring
• Uses standard 24-well cell culture inserts
• Non-invasive and label-free

The ECIS® 8-well Transfilter Array (8wTFA) allows researchers to measure transepithelial electrical resistance (TEER) on standard cell culture 24-well inserts using Electric Cell-substrate Impedance Sensing (ECIS®). The 8wTFA can be used with the ECIS® Z-Theta 16-well station (see above) and can monitor TEER continuously in real-time without having to remove the cells from the incubator.